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Porphyromonas gulae 41-kDa fimbriae induced osteoclast differentiation and cytokine production Porphyromonas gulae 線毛による破骨細胞分化誘導とサイトカイン産生について 佐々木, 悠 ササキ, ハルカ Sasaki, Haruka open access Alveolar Bone Loss Animals Bacteroidaceae Infections Cell Differentiation Cell Line Cytokines Fimbriae Proteins Gene Expression Regulation, Bacterial Macrophages Mice Mice, Inbred BALB C Osteoclasts Porphyromonas Rats Rats, Sprague-Dawley Specific Pathogen-Free Organisms 2014 application/pdf Porphyromonas gulae is considered to be associated with canine periodontitis. We have previously reported that the P. gulae American Type Culture Collection (ATCC) 51700 comprised 41-kDa fimbriae. The purpose of the present study was to demonstrate the roles of 41-kDa fimbrial protein in periodontal disease. In this study, we examined the involvement of the 41-kDa fimbrial protein in osteoclast differentiation and cytokine production in murine macrophages. Furthermore, alveolar bone resorption induced by P. gulae infection in rats was evaluated. To estimate osteoclast differentiation, bone marrow cells and MC3T3-G2/PA6 cells were cultured with or without the 41-kDa fimbrial protein for 7 days. BALB/c mouse peritoneal macrophages were stimulated with the 41-kDa fimbrial protein, and the levels of interleukin (IL)-1β and tumor necrosis factor (TNF)-α production were determined by enzyme-linked immunosorbent assay. Osteoclast differentiation was significantly enhanced by treatment with the 41-kDa fimbrial protein in a dose-dependent manner. The total area of pits formed on the dentine slices with osteoclasts incubated with the 41-kDa fimbrial protein was significantly greater than that of the control. The purified 41-kDa fimbrial protein induced IL-1β and TNF-α production in BALB/c mouse peritoneal macrophages after 6 hr of incubation in a dose-dependent manner. The bone loss level in rats infected with P. gulae was significantly higher than that of the sham-infected rats. These results suggest that P. gulae 41-kDa fimbriae play important roles in the pathogenesis of periodontal disease. eng doctoral thesis VoR https://kdu.repo.nii.ac.jp/records/24 Journal of Veterinary Medical Science, 77(3):265-271, 2015. doi: 10.1292/jvms.14-0463 https://www.jstage.jst.go.jp/article/jvms/77/3/77_14-0463/_article J-STAGE http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4383771/ PMC 甲第456号 博士（歯学） 2015-03-17 32703 神奈川歯科大学 https://kdu.repo.nii.ac.jp/record/24/files/kou456_fulltext.pdf application/pdf 1.2 MB 2021-03-16 https://kdu.repo.nii.ac.jp/record/24/files/kou456_summary.pdf application/pdf 203.0 kB 2016-04-13 https://kdu.repo.nii.ac.jp/record/24/files/kou456_thesisreview.pdf application/pdf 252.0 kB 2016-04-13