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P. gingivalis バイオフィルムに対する太陽電池付与酸化チタン半導体電動歯ブラシの有効性 Efficacy of a solar-powered TiO2 semiconductor electric toothbrush on P. gingivalis biofilm 平井, 直樹 2624 ヒライ, ナオキ Hirai, Naoki 2626 Bacterial Load Bacteriological Techniques Biofilms Coloring Agents Cytoplasm Dental Plaque Electrical Equipment and Supplies Gentian Violet Humans Materials Testing Microscopy, Electron, Scanning Porphyromonas gingivalis Semiconductors Solar Energy Time Factors Titanium Toothbrushing Ultraviolet Rays 神奈川歯科大学 博士（歯学） 2015 application/pdf To determine the efficacy of a solar-powered TiO2 semiconductor electric toothbrush on Porphyromonas gingivalis biofilm. P. gingivalis cells were cultivated on sterilized coverslips under anaerobic conditions and were used as a biofilm. To evaluate the efficacy of the solar-powered TiO2 electric toothbrush on the P. gingivalis biofilm, the bacterial cell biofilm coverslips were placed into sterilized phosphate buffered saline (PBS) and brushed for 1 minute. Following mechanical brushing, the coverslips were stained with 1% crystal violet (CV) for 10 seconds at room temperature. The efficacy of P. gingivalis biofilm removal by the solar-powered TiO2 electric toothbrush was measured through the absorbance of the CV-stained solution containing the removed biofilm at 595 nm. The antimicrobial effect of the solar-powered TiO2 semiconductor was evaluated by the P. gingivalis bacterial count in PBS by blacklight irradiation for 0 to 60 minutes at a distance of 7 cm. The electrical current though the solar-powered TiO2 semiconductor was measured by a digital multimeter. The biofilm removal by the solar-powered TiO2 semiconductor was also evaluated by scanning electron microscopy (SEM). The biofilm removal rate of the solar-powered TiO2 electric toothbrush was 90.1 ± 1.4%, which was 1.3-fold greater than that of non-solar-powered electric toothbrushes. The solar-powered TiO2 semiconductor significantly decreased P. gingivalis cells and biofilm microbial activity in a time-dependent manner (P< 0.01). The electrical current passing through the solar-powered TiO2 semiconductor was 70.5 ± 0.1 µA, which was a 27-fold higher intensity than the non-solar-powered brush. SEM analysis revealed that the solar-powered TiO2 semiconductor caused a biofilm disruption and that cytoplasmic contents were released from the microbial cells. doctoral thesis 2016-03-17 VoR application/pdf application/pdf application/pdf 乙第506号 https://kdu.repo.nii.ac.jp/record/964/files/otsu506_fulltext.pdf https://kdu.repo.nii.ac.jp/record/964/files/otsu506_summary.pdf https://kdu.repo.nii.ac.jp/record/964/files/otsu506_thesisreview.pdf https://kdu.repo.nii.ac.jp/records/964 eng American Journal of Dentistry, 28(2):81-84, 2015 open access