{"created":"2023-06-20T15:00:18.799599+00:00","id":24,"links":{},"metadata":{"_buckets":{"deposit":"2dbdc6ec-9da4-4226-afb5-50363dc0d3ea"},"_deposit":{"created_by":10,"id":"24","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"24"},"status":"published"},"_oai":{"id":"oai:kdu.repo.nii.ac.jp:00000024","sets":["6:8"]},"author_link":["69","70"],"item_10006_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Porphyromonas gulae 線毛による破骨細胞分化誘導とサイトカイン産生について","subitem_alternative_title_language":"ja"}]},"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2015-03-17"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"神奈川歯科大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"32703","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）"}]},"item_10006_description_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"2014","subitem_description_type":"Other"}]},"item_10006_description_17":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Porphyromonas gulae is considered to be associated with canine periodontitis. We have previously reported that the P. gulae American Type Culture Collection (ATCC) 51700 comprised 41-kDa fimbriae. The purpose of the present study was to demonstrate the roles of 41-kDa fimbrial protein in periodontal disease. In this study, we examined the involvement of the 41-kDa fimbrial protein in osteoclast differentiation and cytokine production in murine macrophages. Furthermore, alveolar bone resorption induced by P. gulae infection in rats was evaluated. To estimate osteoclast differentiation, bone marrow cells and MC3T3-G2/PA6 cells were cultured with or without the 41-kDa fimbrial protein for 7 days. BALB/c mouse peritoneal macrophages were stimulated with the 41-kDa fimbrial protein, and the levels of interleukin (IL)-1β and tumor necrosis factor (TNF)-α production were determined by enzyme-linked immunosorbent assay. Osteoclast differentiation was significantly enhanced by treatment with the 41-kDa fimbrial protein in a dose-dependent manner. The total area of pits formed on the dentine slices with osteoclasts incubated with the 41-kDa fimbrial protein was significantly greater than that of the control. The purified 41-kDa fimbrial protein induced IL-1β and TNF-α production in BALB/c mouse peritoneal macrophages after 6 hr of incubation in a dose-dependent manner. The bone loss level in rats infected with P. gulae was significantly higher than that of the sham-infected rats. These results suggest that P. gulae 41-kDa fimbriae play important roles in the pathogenesis of periodontal disease.","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第456号"}]},"item_10006_heading_21":{"attribute_name":"見出し","attribute_value_mlt":[{"subitem_heading_headline":"PDF/A","subitem_heading_language":"ja"}]},"item_10006_relation_14":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Journal of Veterinary Medical Science, 77(3):265-271, 2015. doi: 10.1292/jvms.14-0463"}]}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"J-STAGE"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.jstage.jst.go.jp/article/jvms/77/3/77_14-0463/_article","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"PMC"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4383771/","subitem_relation_type_select":"URI"}}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open 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Text（全文）","objectType":"fulltext","url":"https://kdu.repo.nii.ac.jp/record/24/files/kou456_fulltext.pdf"},"version_id":"bf653d53-05bb-4e7d-a872-79e2056c9966"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-04-13"}],"displaytype":"detail","filename":"kou456_summary.pdf","filesize":[{"value":"203.0 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"論文内容要旨","url":"https://kdu.repo.nii.ac.jp/record/24/files/kou456_summary.pdf"},"version_id":"66f99d3c-ee88-4331-bf06-fdbe14706685"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-04-13"}],"displaytype":"detail","filename":"kou456_thesisreview.pdf","filesize":[{"value":"252.0 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"論文審査要旨、最終審査の結果の要旨","url":"https://kdu.repo.nii.ac.jp/record/24/files/kou456_thesisreview.pdf"},"version_id":"f207cffe-6c10-4999-ac7f-c8fa213086fe"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Alveolar Bone Loss","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Animals","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Bacteroidaceae Infections","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell Differentiation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell Line","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cytokines","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Fimbriae Proteins","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Gene Expression Regulation, Bacterial","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Macrophages","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Mice","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Mice, Inbred BALB C","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Osteoclasts","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Porphyromonas","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Rats","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Rats, Sprague-Dawley","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Specific Pathogen-Free Organisms","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Porphyromonas gulae 41-kDa fimbriae induced osteoclast differentiation and cytokine production","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Porphyromonas gulae 41-kDa fimbriae induced osteoclast differentiation and cytokine production","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"10","path":["8"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2016-04-13"},"publish_date":"2016-04-13","publish_status":"0","recid":"24","relation_version_is_last":true,"title":["Porphyromonas gulae 41-kDa fimbriae induced osteoclast differentiation and cytokine production"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2023-12-21T01:24:22.778908+00:00"}