{"created":"2023-06-20T15:00:18.866191+00:00","id":25,"links":{},"metadata":{"_buckets":{"deposit":"2a9b3690-47a5-48bb-9243-aa61510eed93"},"_deposit":{"created_by":10,"id":"25","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"25"},"status":"published"},"_oai":{"id":"oai:kdu.repo.nii.ac.jp:00000025","sets":["6:8"]},"author_link":["75","76"],"item_10006_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Rhoキナーゼ阻害剤ファスジルは，CXCL14/BRAKの誘導を介して口腔扁平上癌の進展を抑制する","subitem_alternative_title_language":"ja"}]},"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2015-03-17"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"神奈川歯科大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"32703","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）"}]},"item_10006_description_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"2014","subitem_description_type":"Other"}]},"item_10006_description_17":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"CXCL14/BRAK (BRAK) is a secreted chemokine with anti-tumor activity, and its expression is suppressed in tumor cells. We previously reported the anti-tumor activity of BRAK in cell lines of head and neck squamous cell carcinoma (HNSCC) and the suppression of BRAK secretion in these cells. BRAK secretion in fibrosarcoma cells is restored by Fasudil, which is a Rho-kinase (ROCK) inhibitor. In this study, we examined the anti-tumor effect of BRAK by evaluating its gene expression and protein secretion in HNSCC cell lines. We found that BRAK mediated the suppressive effect of Fasudil against HNSCC cells. Tumor development in female BALB/cAJclnu/nu mice was suppressed by Fasudil. Also secretion of BRAK protein by tumor cell lines in vitro was significantly stimulated by Fasudil treatment. Similarly, the production of BRAK protein was significantly increased by the addition of Fasudil to cultured tumor cells. Furthermore Fasudil significantly increased BRAK gene expression at the mRNA level in HNSCC cell line. Inhibition of the RhoA/ROCK pathway by siRNAs significantly stimulated BRAK gene expression. These results show that the tumor-suppressive effect of Fasudil was mediated by BRAK, suggesting that Fasudil may therefore be useful for the treatment of HNSCC.","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第457号"}]},"item_10006_heading_21":{"attribute_name":"見出し","attribute_value_mlt":[{"subitem_heading_headline":"PDF/A","subitem_heading_language":"ja"}]},"item_10006_relation_14":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Biomedical Research, 35(6):381-388, 2014. doi: 10.2220/biomedres.35.381"}]}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"J-STAGE"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.jstage.jst.go.jp/article/biomedres/35/6/35_381/_article","subitem_relation_type_select":"URI"}}]},"item_10006_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"CC BY-NC-ND"}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"本橋, 一彦","creatorNameLang":"ja"},{"creatorName":"モトハシ, カズヒコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"75","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Motohashi, Kazuhiko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"76","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-04-14"}],"displaytype":"detail","filename":"kou457_fulltext.pdf","filesize":[{"value":"3.3 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"Full Text（全文）","objectType":"fulltext","url":"https://kdu.repo.nii.ac.jp/record/25/files/kou457_fulltext.pdf"},"version_id":"49ff86bd-fc3e-43cd-85ab-5bac3d653c67"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-04-14"}],"displaytype":"detail","filename":"kou457_summary.pdf","filesize":[{"value":"135.5 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Cell","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell Line, Tumor","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Chemokines, CXC","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Female","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Gene Expression Regulation, Neoplastic","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Head and Neck Neoplasms","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Humans","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Mice","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Mice, Inbred BALB C","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Neoplasms, Experimental","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Protein Kinase Inhibitors","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"RNA, Messenger","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Real-Time Polymerase Chain Reaction","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"rho-Associated Kinases","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Fasudil, a Rho kinase inhibitor, suppresses tumor growth by inducing CXCL14/BRAK in head and neck squamous cell carcinoma","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Fasudil, a Rho kinase inhibitor, suppresses tumor growth by inducing CXCL14/BRAK in head and neck squamous cell carcinoma","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"10","path":["8"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2016-04-14"},"publish_date":"2016-04-14","publish_status":"0","recid":"25","relation_version_is_last":true,"title":["Fasudil, a Rho kinase inhibitor, suppresses tumor growth by inducing CXCL14/BRAK in head and neck squamous cell carcinoma"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2023-12-21T01:24:23.678173+00:00"}