{"created":"2023-06-20T15:01:19.966089+00:00","id":877,"links":{},"metadata":{"_buckets":{"deposit":"514bbacc-2128-42cc-8b66-4f4d0122200c"},"_deposit":{"created_by":10,"id":"877","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"877"},"status":"published"},"_oai":{"id":"oai:kdu.repo.nii.ac.jp:00000877","sets":["16:70"]},"author_link":["2288","2284","2292","2282","2281","2283","2285","2289","2290","2286","2287","2291"],"item_1692944020530":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"湘南短期大学","subitem_publisher_language":"ja"}]},"item_3_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-03-20","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"35","bibliographicPageStart":"28","bibliographicVolumeNumber":"19","bibliographic_titles":[{"bibliographic_title":"湘南短期大学紀要","bibliographic_titleLang":"ja"},{"bibliographic_title":"Journal of Shonan Junior College","bibliographic_titleLang":"en"}]}]},"item_3_description_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"HGKとHPLFにおけるanion exchanger(AE)の発現状態、ファミリーのタイプ、分布、などを観察し、更に、H_2O_2、ニコチンを用いてAEへの酸化刺激の影響を観察した。その結果、(1)ヒト抗AET、AE2抗体を用いて蛍光抗体法、RT-PCR法により観察した結果、両方法共に、両細胞上にAE2の存在が観察された。(2)AE2の分布を蛍光抗作法で観察した結果、蛍光は、HGKでは、細胞核周辺に多く、HPLFでは、細胞全体に観察された。(3)H_2O_2、ニコチン刺激によるHGK、HPLFの形態変化を観察した結果、H_2O_2刺激でHGKはH_2O_2濃度と作用時間に依存して、細胞間に隙間が出来、核を残す形で細飽が溶解した。HPLFは低濃度刺激では、細胞表面に網目構造を形成して次第に細胞が細くなり、細胞間に隙間を生じ、次第に核を残す形で溶解した。ニコチン刺激でも同様の結果が観察された。(3)RT-PCRを用いてH_2O_2、ニコチン刺激によるAE2への影響を観察した結果、両細胞共に、細胞が破壊寸前までAE2の存在が観察された。(4)H_2O_2、ニコチン刺激による細胞死を観察した結果、両細胞共に、細胞破壊の直前で、apoptosisが観察された。これらの結果より、HGK、HPLFのAEはAE2であり、AE2は酸化刺激より細胞を守り、細胞が破壊される直前まで機能していることが推測できる。","subitem_description_type":"Other"}]},"item_3_description_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"Since periodontal ligament fibroblasts and gingival keratinocytes are closely related to periodontal diseases among human cells constituting periodontal connective tissue, we clarified the presence, distribution and types of AE in human gingival keratinocytes and periodontal ligament fibroblasts. In addition, oxidative stress-induced dynamics of anion exchanger were investigated. The presence of AE on human gingival keratinocytes and periodontal ligament fibroblasts was investigated by the fluorescence antibody method and the RT-PCR method using anti-anion exchanger 1 (AE1) antibody and anti-anion exchanger 2 (AE2) antibody. In both human gingival keratinocytes and periodontal ligament fibroblasts, no expression of AE1 was detected by either method, while AE2 was expressed. The distribution of AE2 was investigated by the flurescence antibody method, and fluorescence was detected on the circumference of nuclear of human gingival keratinocytes, and entire cell body of human periodontal ligament fibroblasts. H_2O_2- and nicotine-induced dynamics of AE2 on human gingival keratinocytes and periodontal ligament fibroblasts were investigated by the RT-PCR method. No changes were observed after stimulation with H_2O_2 or nicotine, but AE was present until the cells were destroyed and lysed by stimulation with H_2O_2 and nicotine. These findings suggested that AE present on human gingival keratinocytes and human periodontal ligament fibroblasts is AE2, and AE2 protect the cell from oxidative stress.","subitem_description_type":"Other"}]},"item_3_description_15":{"attribute_name":"表示順","attribute_value_mlt":[{"subitem_description":"8","subitem_description_type":"Other"}]},"item_3_description_16":{"attribute_name":"アクセション番号","attribute_value_mlt":[{"subitem_description":"KJ00005073921","subitem_description_type":"Other"}]},"item_3_description_2":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P","subitem_description_type":"Other"}]},"item_3_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.18924/00000871","subitem_identifier_reg_type":"JaLC"}]},"item_3_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN10324017","subitem_source_identifier_type":"NCID"}]},"item_3_text_6":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_value":"湘南短期大学歯科衛生学科 "},{"subitem_text_value":" 湘南短期大学歯科衛生学科 "},{"subitem_text_value":" 自治医科大学口腔外科学教室 "},{"subitem_text_value":" 神奈川歯科大学口腔病理学教室 "},{"subitem_text_value":" 神奈川歯科大学生体工学教室 "},{"subitem_text_value":" 北海道大学大学院獣医学研究科臨床分子生物学教室"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"西口, 栄子","creatorNameLang":"ja"},{"creatorName":"ニシグチ, エイコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"2281","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"金子, 和美","creatorNameLang":"ja"},{"creatorName":"カネコ, カズミ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"金子","familyNameLang":"ja"},{"familyName":"カネコ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"和美","givenNameLang":"ja"},{"givenName":"カズミ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"2282","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"神部, 芳則","creatorNameLang":"ja"},{"creatorName":"ジンブ, 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ケイイチ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"槻木","familyNameLang":"ja"},{"familyName":"ツキノキ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"恵一","givenNameLang":"ja"},{"givenName":"ケイイチ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"2284","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"川瀬, 俊夫","creatorNameLang":"ja"},{"creatorName":"カワセ, 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ムツミ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"稲葉","familyNameLang":"ja"},{"familyName":"イナバ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"睦","givenNameLang":"ja"},{"givenName":"ムツミ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"2286","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nishiguchi, Eiko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2287","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Kaneko, Kazumi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Kaneko","familyNameLang":"en"}],"givenNames":[{"givenName":"Kazumi","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2288","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Jinbu, Yoshinori","creatorNameLang":"en"}],"familyNames":[{"familyName":"Jinbu","familyNameLang":"en"}],"givenNames":[{"givenName":"Yoshinori","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2289","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Tsukinoki, Keiichi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Tsukinoki","familyNameLang":"en"}],"givenNames":[{"givenName":"Keiichi","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2290","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Kawase, Toshio","creatorNameLang":"en"}],"familyNames":[{"familyName":"Kawase","familyNameLang":"en"}],"givenNames":[{"givenName":"Toshio","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2291","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Inaba, Mutumi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Inaba","familyNameLang":"en"}],"givenNames":[{"givenName":"Mutumi","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2292","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-04-20"}],"displaytype":"detail","filename":"KJ00005073921.pdf","filesize":[{"value":"1.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00005073921.pdf","url":"https://kdu.repo.nii.ac.jp/record/877/files/KJ00005073921.pdf"},"version_id":"417d18c6-2d7b-4488-8a4d-b752514a3d76"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ヒト歯周組織構成細胞におけるanion exchanger(AE)の発現と分布","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ヒト歯周組織構成細胞におけるanion exchanger(AE)の発現と分布","subitem_title_language":"ja"},{"subitem_title":"Expression and distribution of anion exchangers in the human periodontal connective tissues.","subitem_title_language":"en"}]},"item_type_id":"3","owner":"10","path":["70"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2008-01-01"},"publish_date":"2008-01-01","publish_status":"0","recid":"877","relation_version_is_last":true,"title":["ヒト歯周組織構成細胞におけるanion exchanger(AE)の発現と分布"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2024-02-28T07:37:56.728467+00:00"}