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Acetylation of peptides with deuterium labelled acetic anhydride for the fast atom bombardment mass spectrometry
https://doi.org/10.18924/00000224
https://doi.org/10.18924/000002240adf46f6-dad7-499d-a068-348ee2ee8b80
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00000173759.pdf (536.8 kB)
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| Item type | [ELS]紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 1994-12-25 | |||||
| タイトル | ||||||
| タイトル | Acetylation of peptides with deuterium labelled acetic anhydride for the fast atom bombardment mass spectrometry | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| ID登録 | ||||||
| ID登録 | 10.18924/00000224 | |||||
| ID登録タイプ | JaLC | |||||
| 雑誌書誌ID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN10029312 | |||||
| ページ属性 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | P | |||||
| 論文名よみ | ||||||
| タイトル | Acetylation of peptides with deuterium labelled acetic anhydride for the fast atom bombardment mass spectrometry | |||||
| 言語 | en | |||||
| 著者 |
廣井, 哲也
× 廣井, 哲也× 奥山, 典生× HIROI, Tetsuya× OKUYAMA, Tsuneo |
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| 著者所属(英) | ||||||
| en | ||||||
| Industrial Research Institute of Kanagawa Prefecture, Showa-machi, Kanazawa-ku | ||||||
| 著者所属(英) | ||||||
| en | ||||||
| Kanagawa Dental College | ||||||
| 抄録(英) | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The specific and simplified acetylation method for the α-amino group of a peptide with deuterium labelled anhydride mixture was studied, using some model peptides. The method devised for the N-terminus analysis of a peptide by fast atom bomberdment (FAB) mass spectrometry. The Nterminal α-amino groups of peptides, Tyr-Gly-Gly-Phe-Leu and Ala-Lys-Asn-Phe-Phe, could be acetylated specifically at the range between pH 4.0 and pH 7.0. Below pH 3.0,no acetylation of the peptides were observed. The ionic species of the buffer solutions were also effective on the yields of acetylation of the peptides. The acetylation products were analyzed by HPLC and further by FAB mass spectrometry. With combination of these acetylation technique, it was possible to distinguish between the N-terminal fragment ions and the C-terminal fragment ions on FAB mass spectra. | |||||
| 書誌情報 |
ja : 基礎科学論集 : 教養課程紀要 en : Bulletin of liberal arts and science 巻 12, p. 57-64, 発行日 1994 |
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| 出版者 | ||||||
| 出版者 | 神奈川歯科大学 | |||||
| 言語 | ja | |||||
| 表示順 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 8 | |||||
| アクセション番号 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | KJ00000173759 | |||||
