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CHROMATOGRAPHY OF PROTEIN ON HYDROXYAPATITE IMPREGNATED PAPER
https://doi.org/10.18924/00000227
https://doi.org/10.18924/00000227dd6ff69a-2bd5-424c-92d0-9e200703cd80
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00000173756.pdf (703.8 kB)
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| Item type | [ELS]紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 1994-12-25 | |||||
| タイトル | ||||||
| タイトル | CHROMATOGRAPHY OF PROTEIN ON HYDROXYAPATITE IMPREGNATED PAPER | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| ID登録 | ||||||
| ID登録 | 10.18924/00000227 | |||||
| ID登録タイプ | JaLC | |||||
| 雑誌書誌ID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN10029312 | |||||
| ページ属性 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | P | |||||
| 論文名よみ | ||||||
| タイトル | CHROMATOGRAPHY OF PROTEIN ON HYDROXYAPATITE IMPREGNATED PAPER | |||||
| 言語 | en | |||||
| 著者 |
佐藤, 利明
× 佐藤, 利明× 吉田, 芳哉× 中山, 洋× 小川, 哲朗× 奥山, 典生× Sato, Toshiaki× Yoshida, Yoshiya× Nakayama, Hiroshi× Ogawa, Tetsuro× Okuyama, Tsuneo |
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| 著者所属(英) | ||||||
| en | ||||||
| Division of Virology, Kanagawa Prefectural Pubric Health Laboratry | ||||||
| 著者所属(英) | ||||||
| en | ||||||
| Division of Virology, Kanagawa Prefectural Pubric Health Laboratry | ||||||
| 著者所属(英) | ||||||
| en | ||||||
| Department of Chemistry, Facalty of Science, Tokyo Metropolitan University | ||||||
| 著者所属(英) | ||||||
| en | ||||||
| Asahi Optical Industry Co. Ltd. | ||||||
| 著者所属(英) | ||||||
| en | ||||||
| Department of Dental biotechnology and bioengineering, Kanagawa Dental College | ||||||
| 抄録(英) | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Ceramic hydroxyapatite beads for high performance liquid chromatography (HPLC) was already reported to be a cation exchanger in type and proteins were adsorped and eluted with changing the concentration of sodium phosphate buffer solution. Hydroxyapatite crystalline impregnated paper (HAP paper) which was made of micro crystalline and paper fiber, but without sintering process was used for filtration or separation of biological macromolecule materials. A circular sheet of HAP paper was cut into a suitable size and (0.76mm I. D.) and placed in a column for HPLC of protein. Chromatography was performed across the HAP paper sheet. One sheet of HAP paper could adsorbed about 12μg cytochrome c. A pile of these sheets could also be used for the chromatography of protein as well as hydroxyapatite beads column. HAP paper was useful not only for the separation of protein but also for the preliminary sample clean up for the HPLC, electrophoresis or various immunochemical treatment. | |||||
| 書誌情報 |
ja : 基礎科学論集 : 教養課程紀要 en : Bulletin of liberal arts and science 巻 12, p. 79-86, 発行日 1994 |
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| 出版者 | ||||||
| 出版者 | 神奈川歯科大学 | |||||
| 言語 | ja | |||||
| 表示順 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 11 | |||||
| アクセション番号 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | KJ00000173756 | |||||
