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Comparison of hard tissues that are useful for DNA analysis in forensic autopsy
https://kdu.repo.nii.ac.jp/records/946
https://kdu.repo.nii.ac.jp/records/94680b7fa42-43db-481a-8c3e-db6eeb058c07
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Full Text（全文） (4.6 MB)
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論文内容要旨 (130.1 kB)
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論文審査要旨、最終審査の結果の要旨 (170.2 kB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2016-07-06 | |||||
| タイトル | ||||||
| タイトル | Comparison of hard tissues that are useful for DNA analysis in forensic autopsy | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | DNA typing | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Forensic autopsy | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Hard tissues | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
| 資源タイプ | doctoral thesis | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| その他（別言語等）のタイトル | ||||||
| その他のタイトル | 司法解剖におけるDNA鑑定のための硬組織の有用性の比較検討 | |||||
| 言語 | ja | |||||
| 著者 |
金子, 悠
× 金子, 悠× Kaneko, Yu |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Forensic analysis of DNA from hard tissues can be important when investigating a variety of cases resulting from mass disaster or criminal cases. This study was conducted to evaluate the most suitable tissues, method and sample size for processing of hard tissues prior to DNA isolation. We also evaluated the elapsed time after death in relation to the quantity of DNA extracted. Samples of hard tissues (37 teeth, 42 skull, 42 rib, and 39 nails) from 42 individuals aged between 50 and 83 years were used. The samples were taken from remains following forensic autopsy (from 2 days to 2 years after death). To evaluate the integrity of the nuclear DNA isolated, the percentage of allele calls for short tandem repeat profiles were compared between the hard tissues. DNA typing results indicated that until 1 month after death, any of the four hard tissue samples could be used as an alternative to teeth, allowing analysis of all of the loci. However, in terms of the sampling site, collection method and sample size adjustment, the rib appeared to be the best choice in view of the ease of specimen preparation. Our data suggest that the rib could be an alternative hard tissue sample for DNA analysis of human remains. | |||||
| 学位名 | ||||||
| 学位名 | 博士（歯学） | |||||
| 学位授与機関 | ||||||
| 学位授与機関識別子Scheme | kakenhi | |||||
| 学位授与機関識別子 | 32703 | |||||
| 学位授与機関名 | 神奈川歯科大学 | |||||
| 学位授与年度 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 2015 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2016-03-17 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 甲第480号 | |||||
| 権利 | ||||||
| 権利情報 | 日本法医学会 | |||||
| 情報源 | ||||||
| 関連名称 | Legal Medicine (Tokyo, Japan), 17(6):547-52, 2015. doi: 10.1016/j.legalmed.2015.07.001 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.sciencedirect.com/science/article/pii/S134462231530002X | |||||
| 関連名称 | ScienceDirect | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 見出し | ||||||
| 小見出し | PDF/A | |||||
| 言語 | ja | |||||
